Bio-Connect

IL2 Antibody (clone JES6-5H4, PE)

LS-C811435
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetIL2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL2 Antibody (clone JES6-5H4, PE)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    JES6-5H4
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF
  • Host
    Rat
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIL2
  • SizePrice
Product group Antibodies
Assay Genie
TargetIL2
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ IL-2 Polyclonal Antibody, Unconjugated (bs-10274R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetIL2
  • SizePrice
Anti-human IL-2 mAb (MT2A91) for WB, unconjugated
Product group Antibodies
Mabtech
ApplicationsWestern Blot
TargetIL2
  • SizePrice
Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIL2
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsELISA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetIL2
  • SizePrice
Anti-IL-2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Other Application
TargetIL2
  • SizePrice