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Western Blot analysis of HT29 cells using IL-2 Polyclonal Antibody
Western Blot analysis of HT29 cells using IL-2 Polyclonal Antibody
Western Blot analysis of HT29 cells using IL-2 Polyclonal Antibody

IL2 Antibody

CSB-PA003024
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetIL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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