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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Anti-LIMK1 Antibody

HPA028064
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLIMK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIMK1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3984
  • Target name
    LIMK1
  • Target description
    LIM domain kinase 1
  • Target synonyms
    LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53667
  • Protein Name
    LIM domain kinase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LIMK1 using anti-LIMK1 antibody (PB9716). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Gaster Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: SKOV Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMK1 antigen affinity purified polyclonal antibody (Catalog # PB9716) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMK1 at approximately 72 kDa. The expected band size for LIMK1 is at 72 kDa.
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