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Western Blot analysis of K562 cells using LIMK-1 Polyclonal Antibody
Western Blot analysis of K562 cells using LIMK-1 Polyclonal Antibody
Western Blot analysis of K562 cells using LIMK-1 Polyclonal Antibody

LIMK1 Antibody

CSB-PA003150
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLIMK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    LIMK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3984
  • Target name
    LIMK1
  • Target description
    LIM domain kinase 1
  • Target synonyms
    LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53667
  • Protein Name
    LIM domain kinase 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Western blot using GeneTex Affinity Purified anti-LIM Kinase antibody (GTX10561) shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Signal was detected using standard techniques.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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References
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