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LIMK1 antibody

20R-1981
Biosynth
Product group Antibodies
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    LIMK1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western blot using GeneTex Affinity Purified anti-LIM Kinase antibody (GTX10561) shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Signal was detected using standard techniques.
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References
GeneTex
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
References
Bioss Antibodies
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Product group Antibodies
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