More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/LIMK1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

LIMK1 Polyclonal Antibody

BS-2775R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLIMK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    LIMK1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3984
  • Target name
    LIMK1
  • Target description
    LIM domain kinase 1
  • Target synonyms
    LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53667
  • Protein Name
    LIM domain kinase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1. Zhou J et al., 2014 Oct, Cancer Biol Ther
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bethyl Laboratories
Rabbit anti-LIMK1 Antibody, Affinity PurifiedA302-670A
ApplicationsImmunoPrecipitation
TargetLIMK1
  • SizePrice
Product group Antibodies
Abbexa
LIM Kinase 1 AntibodyABX033701
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
MedChem Express
LIM Kinase 1 Antibody (YA1686, Rabbit)HY-P81941
TargetLIMK1
  • SizePrice
Figure 1. Western blot analysis of LIMK1 using anti-LIMK1 antibody (PB9716). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Gaster Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: U87 Whole Cell Lysate at 40ug, Lane 5: SKOV Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMK1 antigen affinity purified polyclonal antibody (Catalog # PB9716) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMK1 at approximately 72 kDa. The expected band size for LIMK1 is at 72 kDa.
Product group Antibodies
Boster Bio
Anti-LIMK1 Antibody Picoband(r)PB9716-CARRIER-FREE
ApplicationsWestern Blot
TargetLIMK1
  • SizePrice
Western blot using GeneTex Affinity Purified anti-LIM Kinase antibody (GTX10561) shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Signal was detected using standard techniques.
Product group Antibodies
References
GeneTex
LIMK1 antibodyGTX10561
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetLIMK1
  • SizePrice
Western Blot analysis of K562 cells using LIMK-1 Polyclonal Antibody
Product group Antibodies
Cusabio
LIMK1 AntibodyCSB-PA003150
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLIMK1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
LIMK1 / LIMK AntibodyLS-C403296
ApplicationsELISA, ImmunoHistoChemistry
TargetLIMK1
  • SizePrice
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Product group Antibodies
Atlas Antibodies
Anti-LIMK1 AntibodyHPA028064
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetLIMK1
  • SizePrice
Anti-LIMK1 Antibody
Product group Antibodies
Antibodies.com
Anti-LIMK1 AntibodyA97426
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Biosynth
LIMK1 antibody20R-1981
  • SizePrice