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Immunohistochemical staining of human testis shows strong positivity in the nuclear membrane in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong positivity in the nuclear membrane in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong positivity in the nuclear membrane in cells in seminiferous ducts.

Anti-LMNB2 Antibody

HPA062477
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLMNB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMNB2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84823
  • Target name
    LMNB2
  • Target description
    lamin B2
  • Target synonyms
    EPM9, LAMB2, LMN2, MCPH27, lamin-B2, epididymis secretory sperm binding protein, lamin B3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03252
  • Protein Name
    Lamin-B2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (A05348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified polyclonal antibody (Catalog # A05348-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 68 kDa.
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