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Lamin B2 Antibody (YA989, Mouse)

HY-P81283
MedChem Express
TargetLMNB2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Lamin B2 Antibody (YA989, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID84823
  • Target name
    LMNB2
  • Target description
    lamin B2
  • Target synonyms
    EPM9, LAMB2, LMN2, MCPH27, lamin-B2, epididymis secretory sperm binding protein, lamin B3
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Lamin B2 Antibody (YA989) is a mouse-derived non-conjugated IgG1 antibody (Clone NO.: YA989), targeting Lamin B2, with a predicted molecular weight of 70 kDa (observed band size: 70 kDa). Lamin B2 Antibody (YA989) can be used for WB, IP experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (A05348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified polyclonal antibody (Catalog # A05348-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 68 kDa.
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