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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA013005LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA013005LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA013005LA01HU at dilution of 1:100

LMNB2 Antibody

CSB-PA013005LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLMNB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    LMNB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84823
  • Target name
    LMNB2
  • Target description
    lamin B2
  • Target synonyms
    epididymis secretory sperm binding protein; EPM9; LAMB2; lamin B3; lamin-B2; LMN2; MCPH27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03252
  • Protein Name
    Lamin-B2
  • Scientific Description
    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: NIH/3T3 cell lysates probed with Lamin B2 Polyclonal Antibody, Unconjugated (bs-11132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (A05348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified polyclonal antibody (Catalog # A05348-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 68 kDa.
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