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ProductsBack/Mouse anti Lamin B2

Mouse anti Lamin B2

MUB1104P-CE/IVD
Nordic-MUbio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetLMNB2
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Lamin B2
  • Delivery Days Customer
    7
  • Application Supplier Note
    LN43 is suitable for immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Flow Cytometry;Immunohistochemistry (frozen);Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LN43
  • Gene ID84823
  • Target name
    LMNB2
  • Target description
    lamin B2
  • Target synonyms
    EPM9, LAMB2, LMN2, MCPH27, lamin-B2, epididymis secretory sperm binding protein, lamin B3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ03252
  • Protein Name
    Lamin-B2
  • Source
    LN43 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.
  • Reactivity Supplier
    Hamster;Human;Mouse;Swine;Xenopus;Zebrafish
  • UNSPSC
    12352203

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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG. Lane 4: Human plasma. Lane 5: Human Liver tissue. Lane 6: Human Tonsil tissue
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Figure 1. Western blot analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (A05348-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified polyclonal antibody (Catalog # A05348-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 68 kDa.
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