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anti-Mitofusin 1 antibody

ARG58099
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMFN1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-Mitofusin 1 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55669
  • Target name
    MFN1
  • Target description
    mitofusin 1
  • Target synonyms
    hfzo1, hfzo2, mitofusin-1, fzo homolog, mitochondrial transmembrane GTPase FZO-2, mitochondrial transmembrane GTPase Fzo-1, putative transmembrane GTPase, transmembrane GTPase MFN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofusin 1 using anti-Mitofusin 1 antibody (PB9264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD.
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