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Anti-Phospho-PPP1R12A-T853 Antibody

144-50828
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPPP1R12A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Phospho-PPP1R12A-T853 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID4659
  • Target name
    PPP1R12A
  • Target description
    protein phosphatase 1 regulatory subunit 12A
  • Target synonyms
    GUBS, M130, MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A, myosin binding subunit, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A, protein phosphatase myosin-binding subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14974
  • Protein Name
    Protein phosphatase 1 regulatory subunit 12A
  • Scientific Description
    Phospho-PPP1R12A-T853 pAb
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PB9737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 130KD. The expected band size for PPP1R12A is at 130KD.
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