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Anti-POC1A Antibody

144-07465
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPOC1A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-POC1A Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25886
  • Target name
    POC1A
  • Target description
    POC1 centriolar protein A
  • Target synonyms
    PIX2; POC1 centriolar protein homolog A; proteome of centriole protein 1A; SOFT; WD repeat domain 51A; WD repeat-containing protein 51A; WDR51A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBT0
  • Protein Name
    POC1 centriolar protein homolog A
  • Scientific Description
    POC1A Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POC1A using anti-POC1A antibody (A08694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POC1A antigen affinity purified polyclonal antibody (Catalog # A08694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POC1A at approximately 40 kDa. The expected band size for POC1A is at 45 kDa.
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