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Western blot All lanes: POC1A antibody at 2.76ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 40, 41 kDa Observed band size: 46, 70 kDa
Western blot All lanes: POC1A antibody at 2.76ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 40, 41 kDa Observed band size: 46, 70 kDa
Western blot All lanes: POC1A antibody at 2.76ug/ml + Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 40, 41 kDa Observed band size: 46, 70 kDa

POC1A Antibody

CSB-PA822753ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPOC1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    POC1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25886
  • Target name
    POC1A
  • Target description
    POC1 centriolar protein A
  • Target synonyms
    PIX2; POC1 centriolar protein homolog A; proteome of centriole protein 1A; SOFT; WD repeat domain 51A; WD repeat-containing protein 51A; WDR51A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBT0
  • Protein Name
    POC1 centriolar protein homolog A
  • Scientific Description
    Plays an important role in centriole assembly and/or stability and ciliogenesis. Involved in early steps of centriole duplication, as well as in the later steps of centriole length control. Acts in concert with POC1B to ensure centriole integrity and proper mitotic spindle formation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POC1A using anti-POC1A antibody (A08694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POC1A antigen affinity purified polyclonal antibody (Catalog # A08694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POC1A at approximately 40 kDa. The expected band size for POC1A is at 45 kDa.
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