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Anti-POC1A Antibody

CAB7465
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPOC1A
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-POC1A Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID25886
  • Target name
    POC1A
  • Target description
    POC1 centriolar protein A
  • Target synonyms
    PIX2, SOFT, WDR51A, POC1 centriolar protein homolog A, WD repeat domain 51A, WD repeat-containing protein 51A, proteome of centriole protein 1A
  • Host
    Rabbit
  • Protein IDQ8NBT0
  • Protein Name
    POC1 centriolar protein homolog A
  • Scientific Description
    POC1A Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POC1A using anti-POC1A antibody (A08694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POC1A antigen affinity purified polyclonal antibody (Catalog # A08694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POC1A at approximately 40 kDa. The expected band size for POC1A is at 45 kDa.
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