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Anti-Renin Receptor Antibody

Anti-Renin Receptor Antibody

A9906
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Renin Receptor Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to Renin Receptor.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Renin receptor/ATP6AP2 using anti-Renin receptor/ATP6AP2 antibody (A02665-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Renin receptor/ATP6AP2 antigen affinity purified polyclonal antibody (Catalog # A02665-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Renin receptor/ATP6AP2 at approximately 49 kDa. The expected band size for Renin receptor/ATP6AP2 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Renin receptor/ATP6AP2 Antibody Picoband(r)A02665-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetATP6AP2
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Product group Antibodies
RayBiotech
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Western blot All lanes: Renin receptor antibody at 2ug/ml Lane 1: Hela whole cell lysate Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 36 kDa Observed band size: 40 kDa
Product group Antibodies
Cusabio
ATP6AP2 AntibodyCSB-PA002384ESR2HU
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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic/ membranous positivity in neurons and moderately stained neuropil.
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Goat anti-ATP6IP2 / Renin receptor AntibodyEB06118
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Rat
TargetATP6AP2
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Product group Antibodies
LifeSpan BioSciences
ATP6AP2 / Renin Receptor AntibodyLS-C404725
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Renin Receptor antibody [N3C3] detects Renin Receptor protein by immunofluorescent analysis. Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Renin Receptor stained by Renin Receptor antibody [N3C3] (GTX114169) diluted at 1:500. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GeneTex
Renin Receptor antibody [N3C3]GTX114169
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
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