Figure 1. Western blot analysis of Synaptophysin using anti-Synaptophysin antibody (PB9409). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody (Catalog # PB9409) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
Figure 1. Western blot analysis of Synaptophysin using anti-Synaptophysin antibody (PB9409). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody (Catalog # PB9409) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
Anti-Synaptophysin/SYP Antibody Picoband(r)
PB9409-IFLUOR647
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetSYP
Overview
- SupplierBoster Bio
- Product NameAnti-Synaptophysin/SYP Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Synaptophysin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID6855
- Target nameSYP
- Target descriptionsynaptophysin
- Target synonymsmajor synaptic vesicle protein P38; MRX96; MRXSYP; synaptophysin; XLID96
- HostRabbit
- IsotypeIgG
- Protein IDP08247
- Protein NameSynaptophysin
- Scientific DescriptionBoster Bio Anti-Synaptophysin/SYP Antibody Picoband® catalog # PB9409. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203