More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-TNF alpha Biotin

Anti-TNF alpha Biotin

1B-688
EXBIO
ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Porcine, Primate
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-TNF alpha Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow cytometry: Intracellular staining.
  • Applications
    Flow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clone ID
    MAb11
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Isotype
    IgG1
  • Scientific Description
    TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
  • Reactivity
    Human, Porcine, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-TNF alpha [cA2 (Infliximab)]Ab00146-10.0
ApplicationsFlow Cytometry, ELISA, Other Application
ReactivityHuman
TargetTNF
  • SizePrice
Anti-human TNF-alpha mAb (MT25C5), unconjugated
Product group Antibodies
Mabtech
Anti-human TNF-alpha mAb (MT25C5), unconjugated3512-3
ApplicationsELISA, ELISpot Assay
ReactivityHuman
TargetTNF
  • SizePrice
Anti-TNF Antibody
Product group Antibodies
Antibodies.com
Anti-TNF AntibodyA101582
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Biosynth
TNF alpha antibody10-T60A
  • SizePrice
Product group Antibodies
RayBiotech
Anti-TNF alpha Antibody130-10279
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTNF
  • SizePrice
Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
Product group Antibodies
Boster Bio
Anti-TNF alpha Antibody Picoband(r)A00002-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTNF
  • SizePrice
Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
References
Bioss
TNF alpha Polyclonal AntibodyBS-10802R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTNF
  • SizePrice
Product group Antibodies
Cusabio
TNF Monoclonal AntibodyCSB-MA080271
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNF
  • SizePrice
Product group Antibodies
Biomatik
Tnf Polyclonal AntibodyCAC07005
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetTNF
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TNF Alpha AntibodyLS-C150600
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTNF
  • SizePrice