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Immunohistochemical staining of human gastrointestinal shows strong cytoplasmic positivity in peripheral ganglion.
Immunohistochemical staining of human gastrointestinal shows strong cytoplasmic positivity in peripheral ganglion.
Immunohistochemical staining of human gastrointestinal shows strong cytoplasmic positivity in peripheral ganglion.

Anti-TRPV2 Antibody

HPA044993
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTRPV2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRPV2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51393
  • Target name
    TRPV2
  • Target description
    transient receptor potential cation channel subfamily V member 2
  • Target synonyms
    osm-9-like TRP channel 2; OTRPC2; transient receptor potential cation channel subfamily V member 2; vanilloid receptor-like protein 1; VRL; VRL1; VRL-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5S1
  • Protein Name
    Transient receptor potential cation channel subfamily V member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: TRPV2 antibody at 4.51ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86 kDa Observed band size: 86 kDa
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Figure 1. Western blot analysis of VRL1/TRPV2 using anti-VRL1/TRPV2 antibody (A02786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRL1/TRPV2 antigen affinity purified polyclonal antibody (Catalog # A02786-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRL1/TRPV2 at approximately 95KD. The expected band size for VRL1/TRPV2 is at 95KD.
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