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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: TRPV2 antibody at 4.51ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: TRPV2 antibody at 4.51ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: TRPV2 antibody at 4.51ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86 kDa Observed band size: 86 kDa

TRPV2 Antibody

CSB-PA897571LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRPV2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRPV2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51393
  • Target name
    TRPV2
  • Target description
    transient receptor potential cation channel subfamily V member 2
  • Target synonyms
    VRL, VRL-1, VRL1, transient receptor potential cation channel subfamily V member 2, OTRPC2, osm-9-like TRP channel 2, vanilloid receptor-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5S1
  • Protein Name
    Transient receptor potential cation channel subfamily V member 2
  • Scientific Description
    Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term (GTX101868) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
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Figure 1. Western blot analysis of VRL1/TRPV2 using anti-VRL1/TRPV2 antibody (A02786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRL1/TRPV2 antigen affinity purified polyclonal antibody (Catalog # A02786-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRL1/TRPV2 at approximately 95KD. The expected band size for VRL1/TRPV2 is at 95KD.
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