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ProductsBack/VRL1 antibody [C2C3], C-term
Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term (GTX101868) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term (GTX101868) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term (GTX101868) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

VRL1 antibody [C2C3], C-term

GTX101868
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRPV2
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Overview

  • Supplier
    GeneTex
  • Product Name
    VRL1 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.03 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51393
  • Target name
    TRPV2
  • Target description
    transient receptor potential cation channel subfamily V member 2
  • Target synonyms
    VRL, VRL-1, VRL1, transient receptor potential cation channel subfamily V member 2, OTRPC2, osm-9-like TRP channel 2, vanilloid receptor-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5S1
  • Protein Name
    Transient receptor potential cation channel subfamily V member 2
  • Scientific Description
    This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sakakibara A, Sakakibara S, Kusumoto J, et al. Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS One. 2017,12(1):e0169723. doi: 10.1371/journal.pone.0169723
    Read this paper

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Figure 1. Western blot analysis of VRL1/TRPV2 using anti-VRL1/TRPV2 antibody (A02786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRL1/TRPV2 antigen affinity purified polyclonal antibody (Catalog # A02786-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRL1/TRPV2 at approximately 95KD. The expected band size for VRL1/TRPV2 is at 95KD.
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