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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells and basal cells in the seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells and basal cells in the seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells and basal cells in the seminiferous ducts.

Anti-TXNRD1 Antibody

HPA001395
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTXNRD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TXNRD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7296
  • Target name
    TXNRD1
  • Target description
    thioredoxin reductase 1
  • Target synonyms
    gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; oxidoreductase; selenoprotein TXNRD1; testis tissue sperm-binding protein Li 46a; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16881
  • Protein Name
    Thioredoxin reductase 1, cytoplasmic
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: TXNRD1 antibody at 5microg/ml Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 60, 66, 61, 55, 68, 51 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of TXNRD1 using anti-TXNRD1 antibody (A01778-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antigen affinity purified polyclonal antibody (Catalog # A01778-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNRD1 at approximately 65 kDa. The expected band size for TXNRD1 is at 71 kDa.
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