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Western blot All lanes: TXNRD1 antibody at 5microg/ml Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 60, 66, 61, 55, 68, 51 kDa Observed band size: 60 kDa
Western blot All lanes: TXNRD1 antibody at 5microg/ml Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 60, 66, 61, 55, 68, 51 kDa Observed band size: 60 kDa
Western blot All lanes: TXNRD1 antibody at 5microg/ml Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 60, 66, 61, 55, 68, 51 kDa Observed band size: 60 kDa

TXNRD1 Antibody

CSB-PA613705LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTXNRD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TXNRD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7296
  • Target name
    TXNRD1
  • Target description
    thioredoxin reductase 1
  • Target synonyms
    gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; oxidoreductase; selenoprotein TXNRD1; testis tissue sperm-binding protein Li 46a; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16881
  • Protein Name
    Thioredoxin reductase 1, cytoplasmic
  • Scientific Description
    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TXNRD1 using anti-TXNRD1 antibody (A01778-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antigen affinity purified polyclonal antibody (Catalog # A01778-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNRD1 at approximately 65 kDa. The expected band size for TXNRD1 is at 71 kDa.
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