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ProductsBack/Thioredoxin Reductase 1 Antibody (YA2315, Rabbit)

Thioredoxin Reductase 1 Antibody (YA2315, Rabbit)

HY-P82570
MedChem Express
TargetTXNRD1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Thioredoxin Reductase 1 Antibody (YA2315, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7296
  • Target name
    TXNRD1
  • Target description
    thioredoxin reductase 1
  • Target synonyms
    GRIM-12, TR, TR1, TRXR1, TXNR, TXNR1, thioredoxin reductase 1, cytoplasmic, KM-102-derived reductase-like factor, gene associated with retinoic and IFN-induced mortality 12 protein, gene associated with retinoic and interferon-induced mortality 12 protein, oxidoreductase, peroxidase TXNRD1, selenoprotein TXNRD1, testis tissue sperm-binding protein Li 46a, thioredoxin reductase GRIM-12, thioredoxin reductase TR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Thioredoxin Reductase 1 Antibody (YA2315) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2315), targeting Thioredoxin Reductase 1, with a predicted molecular weight of 71 kDa (observed band size: 55 kDa). Thioredoxin Reductase 1 Antibody (YA2315) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TXNRD1 using anti-TXNRD1 antibody (A01778-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antigen affinity purified polyclonal antibody (Catalog # A01778-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNRD1 at approximately 65 kDa. The expected band size for TXNRD1 is at 71 kDa.
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