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ProductsBack/APC Anti-Human CD64 Antibody(10.1)

APC Anti-Human CD64 Antibody(10.1)

APC-30119
Wuhan Fine Biotech
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetFCGR1A
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    APC Anti-Human CD64 Antibody(10.1)
  • Delivery Days Customer
    999
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10.1
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID2209
  • Target name
    FCGR1A
  • Target description
    Fc gamma receptor Ia
  • Target synonyms
    CD64, CD64A, FCG1, FCGR1, FCRI, FcgammaRI, IGFR1, high affinity immunoglobulin gamma Fc receptor I, Fc fragment of IgG receptor Ia, Fc fragment of IgG, high affinity Ia, receptor (CD64), Fc fragment of IgG, high affinity Ia, receptor for (CD64), Fc-gamma RI, Fc-gamma receptor I A1, IgG Fc receptor I, fc-gamma RIA, fcgammaRIa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12314
  • Protein Name
    High affinity immunoglobulin gamma Fc receptor I
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FCGR1A using anti-FCGR1A antibody (A02428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR1A at approximately 70 kDa. The expected band size for FCGR1A is at 43 kDa.
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