![Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20220225_WB_plasma_w_23061202_472.webp)
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Apolipoprotein E antibody [GT27711]
GTX635889
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetAPOE
Overview
- SupplierGeneTex
- Product NameApolipoprotein E antibody [GT27711]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT27711
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID348
- Target nameAPOE
- Target descriptionapolipoprotein E
- Target synonymsAD2, APO-E, ApoE4, LDLCQ5, LPG, apolipoprotein E, apolipoprotein E3
- HostMouse
- IsotypeIgG2a
- Protein IDP02649
- Protein NameApolipoprotein E
- Scientific DescriptionThe protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20200122_ELISA_PAIR_w_23061202_535.webp)
![Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20220225_WB_R_plasma_w_23061202_810.webp)
![Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E antibody [GT27711] (GTX635889) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody. Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E antibody [GT27711] (GTX635889) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20200122_ELISA_Indirect_w_23061202_201.webp)
![Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20250613_IHC-P_R_Multiple_RPKM_1_25062520_809.webp)
![Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20250613_IHC-P_R_Multiple_RPKM_2_25062520_966.webp)
![Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20250704_IHC-P_M_Multiple_RPKM_25072423_462.webp)
![Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20250725_IHC-P_Multiple_RPKM_2_25073123_427.webp)
![Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Apolipoprotein E antibody [GT27711] detects Apolipoprotein E protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. Apolipoprotein E stained by Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20250725_IHC-P_Multiple_RPKM_1_25073123_230.webp)



![Sandwich ELISA analysis of monkey apoE protein using GTX02906 Apolipoprotein E antibody [E981] as coating antibody and GTX02905-02 Apolipoprotein E antibody [E887] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02905-02/GTX02905-02_20210507_ELISA_4_w_23053123_138.webp)
![Sandwich ELISA analysis of monkey apoE protein using GTX02906 Apolipoprotein E antibody [E981] as coating antibody and GTX02905-02 Apolipoprotein E antibody [E887] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02906/GTX02906_20210507_ELISA_w_23053123_676.webp)
![Sandwich ELISA analysis of monkey apoE protein using GTX02906 Apolipoprotein E antibody [E981] as coating antibody and GTX02905-02 Apolipoprotein E antibody [E887] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03051/GTX03051_20210507_ELISA_w_23053123_346.webp)
![Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100053/GTX100053_43006_20171220_ICC_IF_w_23053123_454.webp)

![FACS analysis of HepG2 cells using GTX60446 Apolipoprotein E antibody [1H4]. Green : Apolipoprotein E Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60446/GTX60446_20170912_FACS_w_23061123_124.webp)