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Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX130238) diluted at 1:1000.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX130238) diluted at 1:1000.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX130238) diluted at 1:1000.

Aurora B antibody

GTX130238
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAURKB
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aurora B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.38 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9212
  • Target name
    AURKB
  • Target description
    aurora kinase B
  • Target synonyms
    AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2, aurora kinase B, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora- and Ipl1-like midbody-associated protein 1, aurora- and Ipl1-like midbody-associated protein 1 homolog, aurora-1, aurora-B, aurora-related kinase 2, aurora/IPL1-related kinase 2, protein phosphatase 1, regulatory subunit 48, serine/threonine kinase 12, serine/threonine-protein kinase 12, serine/threonine-protein kinase 5, serine/threonine-protein kinase aurora-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GD4
  • Protein Name
    Aurora kinase B
  • Scientific Description
    Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora B using anti-Aurora B antibody (M00762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora B antigen affinity purified monoclonal antibody (Catalog # M00762-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora B at approximately 39 kDa. The expected band size for Aurora B is at 39 kDa.
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