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Western Blot of Rabbit Anti-Beta Actin Antibody (GTX21801). Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 microg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4oC. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none
Western Blot of Rabbit Anti-Beta Actin Antibody (GTX21801). Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 microg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4oC. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none
Western Blot of Rabbit Anti-Beta Actin Antibody (GTX21801). Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 microg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4oC. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none

beta Actin antibody

GTX21801
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACTB
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Actin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:4000. ICC/IF: 1:500-1:2000. ELISA: 1:10000-1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID60
  • Target name
    ACTB
  • Target description
    actin beta
  • Target synonyms
    BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8, actin, cytoplasmic 1, I(2)-actin, PS1TP5-binding protein 1, beta cytoskeletal actin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60709
  • Protein Name
    Actin, cytoplasmic 1
  • Scientific Description
    The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Up-regulation of histamine H4 receptors contributes to splenic apoptosis in septic mice: counteraction of the antiapoptotic action of nuclear factor-kappaB. Matsuda N et al., 2010 Mar, J Pharmacol Exp Ther
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