F-Actin antibody [NH3] detects F-Actin protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: F-Actin protein stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250. Green: NF-M, stained by NF-M antibody (GTX85461) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
F-Actin antibody [NH3] detects F-Actin protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: F-Actin protein stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250. Green: NF-M, stained by NF-M antibody (GTX85461) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
F-Actin antibody [NH3]
GTX20205
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetACTB
Overview
- SupplierGeneTex
- Product NameF-Actin antibody [NH3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.
- Application Supplier NoteWB: 1/100-1/500. ICC/IF: 1:100-1:1000. FACS: 1/10. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDNH3
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID60
- Target nameACTB
- Target descriptionactin beta
- Target synonymsactin, cytoplasmic 1; beta cytoskeletal actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1
- HostMouse
- IsotypeIgM
- Scientific DescriptionThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Leptin induces endothelial cell migration through Akt, which is inhibited by PPARgamma-ligands. Goetze S et al., 2002 Nov, HypertensionRead more