beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
![beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20160830_IHC-Fr_w_23061202_696.webp "beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).")
![beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500. Blue: DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20150625_IHC_M_w_23061202_499.webp "beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500. Blue: DAPI")
![beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20161004_IFA_w_23061202_507.webp "beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).")
![beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20160808_1_IHC-Fr_w_23061202_940.webp "beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).")
![Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20191122_WB_M_dorsal_root_ganglion_w_23061202_747.webp "Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836). Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20150625_IP_w_23061202_678.webp "Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836). Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_20170309_WB_KO_watermark_w_23061202_269.webp "Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX631836/GTX631836_41855_42459_IHC-P_R_w_23061202_303.webp "beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
beta Tubulin 3/ Tuj1 antibody [GT11710]
GTX631836
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFish, Human, Mouse, Rat
TargetTUBB3
Overview
- SupplierGeneTex
- Product Namebeta Tubulin 3/ Tuj1 antibody [GT11710]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT11710
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10381
- Target nameTUBB3
- Target descriptiontubulin beta 3 class III
- Target synonymsCDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta-3 chain, class III beta-tubulin, tubulin beta-4 chain, tubulin beta-III, tubulin, beta 3
- HostMouse
- IsotypeIgG2b
- Protein IDQ13509
- Protein NameTubulin beta-3 chain
- Scientific DescriptionThis gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]
- ReactivityFish, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Mo H, Kim J, Kim JY, et al. Intranasal administration of induced pluripotent stem cell-derived cortical neural stem cell-secretome as a treatment option for Alzheimer's disease. Transl Neurodegener. 2023,12(1):50. doi: 10.1186/s40035-023-00384-8Read this paper
- Hosoya M, Kitama T, Shimanuki MN, et al. Distribution of macrophages in the developing cochlea of the common marmoset, a primate model animal. Front Immunol. 2023,14:1229414. doi: 10.3389/fimmu.2023.1229414Read this paper
- Jin C, Wu Y, Zhang H, et al. Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes. Bioeng Transl Med. 2023,8(2):e10448. doi: 10.1002/btm2.10448Read this paper
- Ma PW, Wang WL, Chen JW, et al. Treatment with the Ferroptosis Inhibitor Ferrostatin-1 Attenuates Noise-Induced Hearing Loss by Suppressing Ferroptosis and Apoptosis. Oxid Med Cell Longev. 2022,2022:3373828. doi: 10.1155/2022/3373828Read this paper
- Hosoya M, Fujioka M, Okahara J, et al. Early development of the cochlea of the common marmoset, a non-human primate model. Neural Dev. 2022,17(1):6. doi: 10.1186/s13064-022-00162-8Read this paper
- Kim JY, Mo H, Kim J, et al. Mitigating Effect of Estrogen in Alzheimer's Disease-Mimicking Cerebral Organoid. Front Neurosci. 2022,16:816174. doi: 10.3389/fnins.2022.816174Read this paper
- Yang Y, Lu W, Ning M, et al. A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1. Commun Biol. 2022,5(1):309. doi: 10.1038/s42003-022-03263-6Read this paper
- Chen JW, Ma PW, Yuan H, et al. mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis. Front Cell Neurosci. 2022,16:803718. doi: 10.3389/fncel.2022.803718Read this paper
- Vajhøj C, Schmid B, Alik A, et al. Establishment of a human induced pluripotent stem cell neuronal model for identification of modulators of A53T α-synuclein levels and aggregation. PLoS One. 2021,16(12):e0261536. doi: 10.1371/journal.pone.0261536Read this paper
- Rey F, Pandini C, Messa L, et al. α-Synuclein antisense transcript SNCA-AS1 regulates synapses- and aging-related genes suggesting its implication in Parkinson's disease. Aging Cell. 2021,20(12):e13504. doi: 10.1111/acel.13504Read this paper
Datasheet
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![ICC/IF analysis of SH-SY-5Y cells using GTX11314 beta Tubulin 3/ Tuj1 antibody [SDL.3D10] at 1:400(pink) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11314/GTX11314_20170605_ICCIF_w_23060500_832.webp)
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![beta Tubulin 3/ Tuj1 antibody [HL1708] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1708] (GTX637307) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637307/GTX637307_T-44774_20221209_ICC_IF_R_22122018_744.webp)
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