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ProductsBack/BMAL1 (ARNTL) Mouse Monoclonal Antibody [Clone ID: OTI1D1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL. Positive lysates LY400474 (100ug) and LC400474 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL. Positive lysates LY400474 (100ug) and LC400474 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL. Positive lysates LY400474 (100ug) and LC400474 (20ug) can be purchased separately from OriGene.

BMAL1 (ARNTL) Mouse Monoclonal Antibody [Clone ID: OTI1D1]

CF501229
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBMAL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    BMAL1 (ARNTL) Mouse Monoclonal Antibody [Clone ID: OTI1D1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1D1
  • Gene ID406
  • Target name
    BMAL1
  • Target description
    basic helix-loop-helix ARNT like 1
  • Target synonyms
    ARNTL, ARNTL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5, basic helix-loop-helix ARNT-like protein 1, ARNT-like protein 1, brain and muscle, PAS domain containing 3, PAS domain-containing protein 3, aryl hydrocarbon receptor nuclear translocator like, aryl hydrocarbon receptor nuclear translocator-like protein 1, bHLH-PAS protein JAP3, basic helix-loop-helix family member e5, basic-helix-loop-helix-PAS orphan MOP3, basic-helix-loop-helix-PAS protein MOP3, brain and muscle ARNT-like 1, class E basic helix-loop-helix protein 5, member of PAS protein 3, member of PAS superfamily 3, mutant basic helix-loop-helix ARNT-like protein 1, testis tissue sperm-binding protein Li 50e
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO00327
  • Protein Name
    Basic helix-loop-helix ARNT-like protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ARNTL mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
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