ProductsBack/Bmpr1A Polyclonal Antibody

Bmpr1A Polyclonal Antibody

CAC08260

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

TargetBMPR1A

Overview

Supplier
Biomatik
Product Name
BMPR1A Antibody
Delivery Days Customer
12
Applications
ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Applications Supplier
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:200-1:500
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID657
Target name
BMPR1A
Target description
bone morphogenetic protein receptor type 1A
Target synonyms
10q23del; activin A receptor, type II-like kinase 3; activin receptor-like kinase 3; ACVRLK3; ALK3; ALK-3; BMP type-1A receptor; BMPR-1A; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292; serine/threonine-protein kinase receptor R5; SKR5
Host
Rabbit
Isotype
IgG
Protein IDP36894
Protein Name
Bone morphogenetic protein receptor type-1A
Scientific Description
The Bmpr1A Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
Reactivity Supplier
Human
Storage Instruction
-20°C,2°C to 8°C
UNSPSC
12352203

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Figure 1. Western blot analysis of BMPR1A using anti-BMPR1A antibody (A01581-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1A antigen affinity purified polyclonal antibody (Catalog # A01581-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1A at approximately 60 kDa. The expected band size for BMPR1A is at 60 kDa.

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$L1 rat brain lysates L2 mouse intestine lysates probed with Anti BMPR1A/CD292 Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 60kD. Observed band size:60kD.\n$