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BMPR1A antibody

GTX113140
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetBMPR1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    BMPR1A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID657
  • Target name
    BMPR1A
  • Target description
    bone morphogenetic protein receptor type 1A
  • Target synonyms
    10q23del; activin A receptor, type II-like kinase 3; activin receptor-like kinase 3; ACVRLK3; ALK3; ALK-3; BMP type-1A receptor; BMPR-1A; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292; serine/threonine-protein kinase receptor R5; SKR5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36894
  • Protein Name
    Bone morphogenetic protein receptor type-1A
  • Scientific Description
    The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Arginine methylation of R81 in Smad6 confines BMP-induced Smad1 signaling. Wu J et al., 2021 Jan-Jun, J Biol Chem
    Read more

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Figure 1. Western blot analysis of BMPR1A using anti-BMPR1A antibody (A01581-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1A antigen affinity purified polyclonal antibody (Catalog # A01581-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1A at approximately 60 kDa. The expected band size for BMPR1A is at 60 kDa.
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