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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002748LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002748LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002748LA01HU at dilution of 1:100

BMPR1A Antibody

CSB-PA002748LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBMPR1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    BMPR1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID657
  • Target name
    BMPR1A
  • Target description
    bone morphogenetic protein receptor type 1A
  • Target synonyms
    10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5, bone morphogenetic protein receptor type-1A, BMP type-1A receptor, activin A receptor, type II-like kinase 3, activin receptor-like kinase 3, bone morphogenetic protein receptor, type IA, serine/threonine-protein kinase receptor R5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36894
  • Protein Name
    Bone morphogenetic protein receptor type-1A
  • Scientific Description
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP-2 and BMP-4. Positively regulates chondrocyte differentiation through GDF5 interaction.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BMPR1A using anti-BMPR1A antibody (A01581-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1A antigen affinity purified polyclonal antibody (Catalog # A01581-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1A at approximately 60 kDa. The expected band size for BMPR1A is at 60 kDa.
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