ProductsBack/Goat anti-BMPR1A

Goat anti-BMPR1A

EB07197

ApplicationsWestern Blot, ELISA

Product group Antibodies

TargetBMPR1A

Overview

Supplier
Everest Biotech
Product Name
Goat anti-BMPR1A Antibody
Delivery Days Customer
5
Applications
Western Blot, ELISA
Applications Supplier
Pep-ELISA, WB
Certification
Research Use Only
Clonality
Polyclonal
Concentration
0.5 mg/ml
Gene ID657
Target name
BMPR1A
Target description
bone morphogenetic protein receptor type 1A
Target synonyms
10q23del; activin A receptor, type II-like kinase 3; activin receptor-like kinase 3; ACVRLK3; ALK3; ALK-3; BMP type-1A receptor; BMPR-1A; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292; serine/threonine-protein kinase receptor R5; SKR5
Host
Goat
Scientific Description
RefSeq number(s): NP_004320.2. Purification: Antigen affinity purified. Names and symbols: BMPR1A; bone morphogenetic protein receptor, type IA ; HGNC:1076; ACVRLK3; ALK3; activin A receptor, type II-like kinase 3
Reactivity Supplier
Human, Dog
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of BMPR1A using anti-BMPR1A antibody (A01581-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1A antigen affinity purified polyclonal antibody (Catalog # A01581-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1A at approximately 60 kDa. The expected band size for BMPR1A is at 60 kDa.

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$L1 rat brain lysates L2 mouse intestine lysates probed with Anti BMPR1A/CD292 Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 60kD. Observed band size:60kD.\n$