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WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein. Red : Primary antibody Green : GAPDH Dilution : 1:2000 Lane 1 : protein marker Lane 2 : HeLa cells grown in FBS free media Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours Lane 4 : rat cortical neurons Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours
WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein. Red : Primary antibody Green : GAPDH Dilution : 1:2000 Lane 1 : protein marker Lane 2 : HeLa cells grown in FBS free media Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours Lane 4 : rat cortical neurons Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours
WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein. Red : Primary antibody Green : GAPDH Dilution : 1:2000 Lane 1 : protein marker Lane 2 : HeLa cells grown in FBS free media Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours Lane 4 : rat cortical neurons Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours

c-Fos antibody

GTX03375
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOS
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Overview

  • Supplier
    GeneTex
  • Product Name
    c-Fos antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01100
  • Protein Name
    Protein c-Fos
  • Scientific Description
    The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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