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ProductsBack/CD11b antibody [M1/70.15] (FITC)

CD11b antibody [M1/70.15] (FITC)

GTX82969
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetItgam
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD11b antibody [M1/70.15] (FITC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    For Blocking: Use at an assay dependent dilution. For FACS: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M1/70.15
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Gene ID16409
  • Target name
    Itgam
  • Target description
    integrin alpha M
  • Target synonyms
    CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, integrin alpha-M, CD11 antigen-like family member B, CD11B (p170), CR-3 alpha chain, Mac-1 alpha, cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement receptor type 3, leukocyte adhesion receptor MO1, macrophage antigen alpha
  • Host
    Rat
  • Isotype
    IgG2b
  • Scientific Description
    Part of a cation efflux system that mediates resistance to copper and silver. Binds one copper per polypeptide.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Nakamura K, Sakai S, Tsuyama J, et al. Extracellular DJ-1 induces sterile inflammation in the ischemic brain. PLoS Biol. 2021,19(5):e3000939. doi: 10.1371/journal.pbio.3000939
    Read this paper
  • Wang HT, Yang LC, Yu HC, et al. Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill. J Food Drug Anal. 2018,26(2):678-687. doi: 10.1016/j.jfda.2017.07.006
    Read this paper
  • Zhang Z, Jyoti A, Balakrishnan B, et al. Trajectory of inflammatory and microglial activation markers in the postnatal rabbit brain following intrauterine endotoxin exposure. Neurobiol Dis. 2018,111:153-162. doi: 10.1016/j.nbd.2017.12.013
    Read this paper
  • Yang LC, Lu TJ, Lin WC. A Type II Arabinogalactan from Anoectochilus formosanus for G-CSF Production in Macrophages and Leukopenia Improvement in CT26-Bearing Mice Treated with 5-Fluorouracil. Evid Based Complement Alternat Med. 2013,2013:458075. doi: 10.1155/2013/458075
    Read this paper

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Figure 1. Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.
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