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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CD133 antibody [N1C1]

GTX102109
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPROM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD133 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8842
  • Target name
    PROM1
  • Target description
    prominin 1
  • Target synonyms
    AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1, AC133 antigen, Retinitis pigmentosa 41, Cone-rod dystrophy 12, antigen AC133, hProminin, hematopoietic stem cell antigen, prominin-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43490
  • Protein Name
    Prominin-1
  • Scientific Description
    This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang BY, Shen HT, Lee YL, et al. Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression. Am J Cancer Res. 2023,13(10):4721-4733.
    Read this paper
  • Huang TH, Wu ATH, Cheng TS, et al. In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer. J Cell Mol Med. 2019,23(12):8184-8195. doi: 10.1111/jcmm.14689
    Read this paper
  • Hong KJ, Wu DC, Cheng KH, et al. RECK inhibits stemness gene expression and tumorigenicity of gastric cancer cells by suppressing ADAM-mediated Notch1 activation. J Cell Physiol. 2014,229(2):191-201. doi: 10.1002/jcp.24434
    Read this paper