![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102109/GTX102109_39549_20190530_WB_w_23060100_233.webp)
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CD133 antibody [N1C1]
GTX102109
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPROM1
Overview
- SupplierGeneTex
- Product NameCD133 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8842
- Target namePROM1
- Target descriptionprominin 1
- Target synonymsAC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1, AC133 antigen, Retinitis pigmentosa 41, Cone-rod dystrophy 12, antigen AC133, hProminin, hematopoietic stem cell antigen, prominin-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDO43490
- Protein NameProminin-1
- Scientific DescriptionThis gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wang BY, Shen HT, Lee YL, et al. Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression. Am J Cancer Res. 2023,13(10):4721-4733.Read this paper
- Huang TH, Wu ATH, Cheng TS, et al. In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer. J Cell Mol Med. 2019,23(12):8184-8195. doi: 10.1111/jcmm.14689Read this paper
- Hong KJ, Wu DC, Cheng KH, et al. RECK inhibits stemness gene expression and tumorigenicity of gastric cancer cells by suppressing ADAM-mediated Notch1 activation. J Cell Physiol. 2014,229(2):191-201. doi: 10.1002/jcp.24434Read this paper
![CD133 antibody [N1C1] detects CD133 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells 7.5 % SDS-PAGE CD133 antibody [N1C1] (GTX102109) dilution: 1:5000 CD133 antibody [N1C1] detects CD133 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells 7.5 % SDS-PAGE CD133 antibody [N1C1] (GTX102109) dilution: 1:5000](https://www.genetex.com/upload/website/prouct_img/normal/GTX102109/GTX102109_39549_WB_B_w_23060100_523.webp)
![CD133 antibody [N1C1] detects CD133 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CD133 stained by CD133 antibody [N1C1] (GTX102109) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min CD133 antibody [N1C1] detects CD133 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CD133 stained by CD133 antibody [N1C1] (GTX102109) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102109/GTX102109_39549_20220325_IHC-P_w_23060100_181.webp)


![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100567/GTX100567_39471_20190530_WB_w_23060100_319.webp)
![IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]](https://www.genetex.com/upload/website/prouct_img/normal/GTX60471/GTX60471_20170912_IHC-P_w_23061123_823.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20220225_WB_w_23061202_503.webp)
![CD133 antibody [HL1631] (GTX637124) detects CD133 protein by flow cytometry analysis. Sample: NT2D1 cell. Gray: Rabbit IgG Isotype control. Red: CD133 antibody [HL1631] (GTX637124) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis. *The competitor (clone [AC133]) is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637124/GTX637124_T-44739_20220722_FACS_22081000_264.webp)