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CD81 Antibody is validated for ELISA, FC, IHC, WB & tested in Human, Mouse, Rat. Cusabio is available at Bio-Connect

CD81 Antibody antibody | CSB-RA004960A0HU | Cusabio

CD81 Recombinant Monoclonal Antibody

Antibodies

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

Western Blot Positive WB detected in:K562 whole cell lysate All lanes:CD81 antibody at 1.25microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa

IHC image of CSB-RA004960A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA004960A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay histogram showing Jurkat cells stained with CSB-RA004960A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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CD81 Recombinant Monoclonal Antibody

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