![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX641351/GTX641351_T-45600_20241122_WB_M_tissue_24112622_154.webp)
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody
Choline Acetyltransferase antibody [HL3466]
GTX641351
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHAT
Overview
- SupplierGeneTex
- Product NameCholine Acetyltransferase antibody [HL3466]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3466
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1103
- Target nameCHAT
- Target descriptioncholine O-acetyltransferase
- Target synonymsCHOACTASE, CMS1A, CMS1A2, CMS6, choline O-acetyltransferase, acetyl CoA:choline O-acetyltransferase, choline acetylase
- HostRabbit
- IsotypeIgG
- Protein IDP28329
- Protein NameCholine O-acetyltransferase
- Scientific DescriptionThis gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimers disease. Polymorphisms in this gene have been associated with Alzheimers disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX641351/GTX641351_T-45600_20241122_WB_24112622_151.webp)
![Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641351/GTX641351_T-45600_20250117_IHC-P_25020422_375.webp)
![Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Paraffin-embedded rat eye. Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Paraffin-embedded rat eye. Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641351/GTX641351_T-45600_20250117_IHC-P_R_25020422_318.webp)
![Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse eye. Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Hoechst 33342 staining. Choline Acetyltransferase antibody [HL3466] detects Choline Acetyltransferase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse eye. Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL3466] (GTX641351) diluted at 1:250. Red: beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641351/GTX641351_T-45600_20250620_IHC-Fr_M_25062520_973.webp)







![Choline Acetyltransferase antibody [HL2014] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [HL2014] (GTX637915) diluted at 1:100. Red: Cytoskeleton, a GTX631836 marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836_41855, 1, 500) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637915/GTX637915_T-44872_20221230_IHC-P_M_1_23020621_361.webp)