Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![cIAP1 antibody [GT422] detects cIAP1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE cIAP1 antibody [GT422] (GTX629402) dilution: 1:250](https://www.genetex.com/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_WB_w_23061202_552.webp "cIAP1 antibody [GT422] detects cIAP1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE cIAP1 antibody [GT422] (GTX629402) dilution: 1:250")
![cIAP1 antibody [GT422] detects cIAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: cIAP1 stained by cIAP1 antibody [GT422] (GTX629402) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_20180321_ICC_IF_w_23061202_980.webp "cIAP1 antibody [GT422] detects cIAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: cIAP1 stained by cIAP1 antibody [GT422] (GTX629402) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10μm.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629402/GTX629402_41344_20181026_WB_shRNA_watermark_w_23061202_179.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [GT422] (GTX629402) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
cIAP1 antibody [GT422]
GTX629402
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetBIRC2
Overview
- SupplierGeneTex
- Product NamecIAP1 antibody [GT422]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT422
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID329
- Target nameBIRC2
- Target descriptionbaculoviral IAP repeat containing 2
- Target synonymsAPI1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1, baculoviral IAP repeat-containing protein 2, IAP homolog B, IAP-2, NFR2-TRAF signalling complex protein, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1, cellular inhibitor of apoptosis 1, inhibitor of apoptosis protein 2
- HostMouse
- IsotypeIgG2a
- Protein IDQ13490
- Protein NameBaculoviral IAP repeat-containing protein 2
- Scientific DescriptionThe protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635979/GTX635979_44235_20220812_WB_competitor_watermark_22081423_651.webp)
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