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Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] (GTX82722).
Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] (GTX82722).
Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~62k COBRA1 protein using COBRA1 (NELF-B) antibody [F7E4] (GTX82722).

COBRA1 antibody [F7E4]

GTX82722
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNELFB
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Overview

  • Supplier
    GeneTex
  • Product Name
    COBRA1 antibody [F7E4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Optimal working dilutions should be determined experimentally by the end user.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F7E4
  • Conjugate
    Unconjugated
  • Gene ID25920
  • Target name
    NELFB
  • Target description
    negative elongation factor complex member B
  • Target synonyms
    COBRA1; cofactor of BRCA1; negative elongation factor B; negative elongation factor protein B; NELF-B
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8WX92
  • Protein Name
    Negative elongation factor B
  • Scientific Description
    NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hey1- and p53-dependent TrkC proapoptotic activity controls neuroblastoma growth. Menard M et al., 2018 May, PLoS Biol
    Read more

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Figure 1. Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
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