Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_42184_20161013_WB_shRNA_watermark_w_23061202_353.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_44517_20211210_WB_w_23061202_287.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cofilin 1 stained by Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_44517_20220218_ICC_IF_w_23061202_420.webp "Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cofilin 1 stained by Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_42184_20160809_IHC-P_M_w_23061202_779.webp "Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] (GTX632582). Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_42184_20160401_IP_w_23061202_225.webp "Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] (GTX632582). Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632582/GTX632582_42184_20170615_WB_R_w_23061202_761.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Cofilin 1 antibody [GT217]
GTX632582
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCFL1
Overview
- SupplierGeneTex
- Product NameCofilin 1 antibody [GT217]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT217
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1072
- Target nameCFL1
- Target descriptioncofilin 1
- Target synonymsCFL, HEL-S-15, cofilin, cofilin-1, 18 kDa phosphoprotein, cofilin 1 (non-muscle), epididymis secretory protein Li 15, p18
- HostMouse
- IsotypeIgG2b
- Protein IDP23528
- Protein NameCofilin-1
- Scientific DescriptionThe protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Cofilin 1 antibody detects Cofilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody (GTX102156) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102156/GTX102156_40051_20150410_IFA_w_23060100_118.webp)
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