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Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (GTX12866, Lane 3 and 4)
Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (GTX12866, Lane 3 and 4)
Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (GTX12866, Lane 3 and 4)

Cofilin 1 (phospho Ser3) antibody

GTX12866
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCFL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cofilin 1 (phospho Ser3) antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1072
  • Target name
    CFL1
  • Target description
    cofilin 1
  • Target synonyms
    18 kDa phosphoprotein; CFL; cofilin; cofilin 1 (non-muscle); cofilin-1; epididymis secretory protein Li 15; HEL-S-15; p18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23528
  • Protein Name
    Cofilin-1
  • Scientific Description
    The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • 14-3-3epsilon and aeta regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brain. Toyo-oka K et al., 2014 Sep 3, J Neurosci
    Read more

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Figure 1. Western blot analysis of Cofilin using anti-Cofilin antibody (PB9033). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9033) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.
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