Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 (phospho Ser3) antibody (GTX133865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 (phospho Ser3) antibody (GTX133865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cofilin 1 (phospho Ser3) antibody
GTX133865
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetCFL1
Overview
- SupplierGeneTex
- Product NameCofilin 1 (phospho Ser3) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.36 mg/ml
- ConjugateUnconjugated
- Gene ID1072
- Target nameCFL1
- Target descriptioncofilin 1
- Target synonymsCFL, HEL-S-15, cofilin, cofilin-1, 18 kDa phosphoprotein, cofilin 1 (non-muscle), epididymis secretory protein Li 15, p18
- HostRabbit
- IsotypeIgG
- Protein IDP23528
- Protein NameCofilin-1
- Scientific DescriptionCofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Cofilin 1 antibody detects Cofilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody (GTX102156) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102156/GTX102156_40051_20150410_IFA_w_23060100_118.webp)
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