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Complement C3 Antibody (clone 11H9)

LS-C22283
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityMouse
TargetC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Complement C3 Antibody (clone 11H9)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. ELISA: Coating antibody. Western Blot: 1:25. Flow Cytometry: 1:25. Immunohistochemistry (Frozen sections): 1:25.. ELISA, Flo (1:25), IHC, IHC-Fr, IP, WB (1:25) Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. ELISA: Coating antibody. Western Blot: 1:25. Flow Cytometry: 1:25. Immunohistochemistry (Frozen sections): 1:25.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    11H9
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID718
  • Target name
    C3
  • Target description
    complement C3
  • Target synonyms
    AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
  • Host
    Rat
  • Isotype
    IgG2a
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C3 using anti-C3 antibody (A00168-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse plasma lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # A00168-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C3 at approximately 128KD/187KD. The expected band size for C3 is at 187KD.
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