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Western Blot analysis of 293 cells using CYP26A1 Polyclonal Antibody
Western Blot analysis of 293 cells using CYP26A1 Polyclonal Antibody
Western Blot analysis of 293 cells using CYP26A1 Polyclonal Antibody

CYP26A1 Antibody

CSB-PA001937
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYP26A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP26A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1592
  • Target name
    CYP26A1
  • Target description
    cytochrome P450 family 26 subfamily A member 1
  • Target synonyms
    CP26, CYP26, P450RAI, P450RAI1, cytochrome P450 26A1, P450, retinoic acid-inactivating, 1, cytochrome P450 retinoic acid-inactivating 1, cytochrome P450, family 26, subfamily A, polypeptide 1, cytochrome P450, subfamily XXVIA, polypeptide 1, cytochrome P450RAI, hP450RAI, retinoic acid 4-hydroxylase, retinoic acid-metabolizing cytochrome
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43174
  • Protein Name
    Cytochrome P450 26A1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP26A1 using anti-CYP26A1 antibody (A03646-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat H9C2(2-1) whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP26A1 antigen affinity purified polyclonal antibody (Catalog # A03646-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP26A1 at approximately 56 kDa. The expected band size for CYP26A1 is at 56 kDa.
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