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Cytochrome P450 26A1 (CYP26A1) Polyclonal Antibody

CAU32510
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityPorcine
TargetCYP26A1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cytochrome P450 26A1 (CYP26A1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1592
  • Target name
    CYP26A1
  • Target description
    cytochrome P450 family 26 subfamily A member 1
  • Target synonyms
    CP26, CYP26, P450RAI, P450RAI1, cytochrome P450 26A1, P450, retinoic acid-inactivating, 1, cytochrome P450 retinoic acid-inactivating 1, cytochrome P450, family 26, subfamily A, polypeptide 1, cytochrome P450, subfamily XXVIA, polypeptide 1, cytochrome P450RAI, hP450RAI, retinoic acid 4-hydroxylase, retinoic acid-metabolizing cytochrome
  • Host
    Rabbit
  • Protein IDO43174
  • Protein Name
    Cytochrome P450 26A1
  • Scientific Description
    The Cytochrome P450 26A1 (CYP26A1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity
    Porcine
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP26A1 using anti-CYP26A1 antibody (A03646-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat H9C2(2-1) whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP26A1 antigen affinity purified polyclonal antibody (Catalog # A03646-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP26A1 at approximately 56 kDa. The expected band size for CYP26A1 is at 56 kDa.
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