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Western Blot analysis of Mouse heart tissue using ECE1 Polyclonal Antibody at dilution of 1:900
Western Blot analysis of Mouse heart tissue using ECE1 Polyclonal Antibody at dilution of 1:900
Western Blot analysis of Mouse heart tissue using ECE1 Polyclonal Antibody at dilution of 1:900

ECE1 Polyclonal Antibody

E-AB-10296
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ECE1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.9mg/ml
  • Scientific Description
    The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ECE1 using anti-ECE1 antibody (A02519-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE1 antigen affinity purified polyclonal antibody (Catalog # A02519-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECE1 at approximately 87KD. The expected band size for ECE1 is at 87KD.
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