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ProductsBack/eIF2 alpha (phospho Ser51) antibody

eIF2 alpha (phospho Ser51) antibody

GTX130006
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetEIF2S1
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF2 alpha (phospho Ser51) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.77 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1965
  • Target name
    EIF2S1
  • Target description
    eukaryotic translation initiation factor 2 subunit alpha
  • Target synonyms
    EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05198
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 1
  • Scientific Description
    The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression. Wang IH et al., 2020 Apr 27, Cancers (Basel)
    Read more

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Figure 1. Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (P04387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified monoclonal antibody (Catalog # P04387) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 36 kDa. The expected band size for EIF2S1 is at 36 kDa.
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